Gel Amaraso Yumuhondo Tube

Gutandukanya gel gukusanya amaraso byakoreshejwe cyane muri laboratoire.Gutandukanya gel birashobora gukora urwego rwo kwigunga hagati yibice bigize selile na serumu (plasma), bikarinda neza guhanahana ibintu hagati yingirangingo zamaraso na serumu (plasma), kandi bikanemeza ko ibice bya serumu (plasma) bihagaze neza mugihe runaka.Gutandukanya kole bigizwe ahanini na rebero ya silicone, hydrocarbone ya macromolecular, hydrophobe glue, nibindi nkibikoresho bya polymer, ntibishobora gushonga mumazi na inert.Nibintu bya thixotropique viscous fluid ifite ubucucike bwa 1.04-1.05 mmol / Hagati ya L, ifite ibyiza byo kurwanya okiside, kurwanya ubushyuhe bwinshi, kurwanya ubushyuhe buke, no gukomera kwumwuka mwiza.Ubucucike bwa serumu ni 1.026-1.031 mmol / L, naho hematocrit ni 1.090-1.095.Bitewe nuburemere bwihariye, gel itandukanya iri hagati ya serumu na selile yamaraso, mubihe rero bisanzwe, amaraso azagaragara muburyo bukurikiranye nyuma ya centrifugation.Serumu, itandukanya gel, na selile yamaraso amagorofa 3.

Muri rusange hari ubwoko bubiri bwo gutandukanya amaraso yo gukusanya amaraso akoreshwa muri laboratoire: serumu itandukanya gel procoagulation tube na plasma yo gutandukanya gel anticoagulation tube.Serumu itandukanya gel procoagulation tube ni ukongeramo coagulant mumiyoboro yo gukusanya amaraso kugirango bigabanye igihe cyo guhuza amaraso, kubona serumu vuba, no gutanga ibisubizo mugihe gito.Ikirahuri cyo gukusanya amaraso yikirahure ntigikeneye kongeramo coagulants, kandi amaraso ahuye nurukuta rwikirahure bizatera coagulation.Ariko, mugihe ibintu bya coagulation XI na XII bihuye nigituba cyo gukusanya amaraso ya plastike, ubushobozi bwabo bwo gukora burakomeye cyane, kandi hagomba kongerwamo coagulant kugirango bigabanye igihe cyo guterana.Umuyoboro wa plasma utandukanya gel anticoagulation watewe hamwe na anticoagulants nka lithium heparin kurukuta rwimbere rwigitereko cyamaraso yo gutandukanya amaraso kugirango gikemurwe byihuse byihutirwa bya plasma biochemiki.

Mubikorwa bifatika, bikunze kugaragara ko ingaruka zo gutandukanya imiyoboro yo gukusanya amaraso yo gutandukanya gel atari nziza, kurugero: mubice bimwe byo gutandukanya reberi, birashobora kugaragara ko ibice bya gel bitandukanya cyangwa ibitonyanga byamavuta bireremba hejuru yubuso serumu cyangwa yahagaritswe muri serumu;gutandukanya gel layer ireremba hejuru ya serumu.hejuru nibindi Gutandukanya geles birashobora kandi kubangamira ibisubizo bimwe byikizamini.Mu ishami ryacu, byagaragaye ko icyiciro cyihariye cya reagents hamwe na serumu yo gutandukanya serumu itandukanya umuyoboro wa reaction hagati yabo mugihe HBSAg yatahuye sisitemu yo gutahura Abbott i2000SR, bikavamo ibisubizo byiza.

Uru rupapuro rusesengura cyane cyane mubice bibiri, ni ukuvuga impamvu zitera ingaruka mbi zo gutandukana kwa gel itandukanya, ningaruka zo kwinjiza gel itandukanya kubipimo.

1. Uburyo bwo gutandukanya serumu na plasma mugutandukanya gel Gutandukanya gel ni thixotropic mucocolloide igizwe na hydrophobique organic organic hamwe nifu ya silika.Imiterere irimo umubare munini wa hydrogène.Kubaho kwa hydrogène bigize ishingiro ryimiti ya thixotropy ya gel itandukanya..Uburemere bwihariye bwa geli itandukanya bugumaho kuri 1.05, uburemere bwihariye bwibigize amazi yamaraso bigera kuri 1.02, naho uburemere bwihariye bwamaraso agizwe na 1.08.Iyo gel itandukanya n'amaraso ya coagulée (cyangwa amaraso yose arwanya anticagulée) yashyizwe mumiyoboro imwe yipimisha, Bitewe ningufu za centrifugal zikoreshwa kuri gel itandukanya, imiyoboro ya hydrogène ihuza imiyoboro imeze nkurunigi, no gutandukana. gel ihinduka amazi-viscosity fluid.Bitewe nuburemere butandukanye bwihariye, gel yo gutandukana irahindurwa kandi igashyirwa mubice bitatu byamaraso (anticoagulated blood blood) / gutandukanya gel / serumu (plasma).Iyo centrifuge ihagaritse kuzunguruka ikabura imbaraga za centrifugal, uduce duto twumunyururu muri gel itandukanya twongera gukora urusobekerane rwumuyoboro wa hydrogène, kugarura imiterere yambere ya gel-viscosity gel, hanyuma igakora urwego rwo kwigunga hagati ya serumu (plasma) hamwe nu maraso (anticoagulated maraso yose)..

2. Impamvu zingaruka zo gutandukana nabi zo gutandukanya gel

2.1 Gutandukanya ubuziranenge bwa Gel Uburemere bwihariye bwa geli itandukanya ni hagati ya serumu (plasma) na selile yamaraso, akaba aribwo shingiro ryumubiri ryo guhinduranya gele itandukanya no gutandukanya serumu (plasma).Niba ubwiza bwa gel yo gutandukanya umuyoboro wamaraso ukennye kandi uburemere bwihariye butujuje ibyangombwa, byanze bikunze bizagira ingaruka zo gutandukanya serumu (plasma), nibintu byerekana ko gel hamwe na serumu (plasma) bitandukanya gufatanya birashoboka.

2Impamvu akenshi nuko amaraso atuzuye neza mbere ya centrifugation.Amaraso atuzuye arashobora gutera fibrin kuvangwa murwego rwo kwigunga.Umuyoboro wa serumu utandukanya reberi ugomba gukoreshwa neza ukurikije amabwiriza, kandi serumu irashobora gutegurwa na centrifugation nyuma yamaraso yuzuye (muri rusange, umuyoboro wa pulasitike urimo coagulant ugomba gushyirwaho neza muminota igera kuri 30, namaraso umuyoboro wo gukusanya udafite coagulant ugomba gushyirwaho neza muminota 60-90).icyitegererezo cyiza cya serumu.

2.3 Ubushyuhe bwa Centrifugation Ubushyuhe bwa centrifugation bugira ingaruka zikomeye ku ngaruka zo gutandukanya serumu nuyoboro wa gel.Serumu yari isobanutse muri inert itandukanya gel yihuta ya coagulation itandukanijwe na centrifuge isanzwe mubushyuhe bwicyumba, ariko amasaro yamavuta yubunini butandukanye yagaragaye muri 15% kugeza kuri 20% byicyitegererezo.Ku rundi ruhande, nta masaro y'amavuta yabonetse muri serumu yatandukanijwe n'umuyoboro w'ikizamini washyizwemo na centrifuge y'ubushyuhe buke.Iyo ubushyuhe burenze ubushyuhe bwububiko busabwa kugirango gel itandukane, gel inert izashonga muri serumu.Ntabwo izahagarika gusa kandi yanduze icyitegererezo cy'urushinge hamwe nigikombe cya reaction ya analyse ya biohimiki, ariko kandi izagira ingaruka nini ugereranije nibisubizo byo gupima ibinyabuzima.