PRP Igituba Acd Tubes
Ibisobanuro bigufi:
Anticoagulant Citrate Dextrose Umuti, bakunze kwita ACD-A cyangwa Umuti A ni igisubizo kitari pyrogene, sterile.Iyi element ikoreshwa nka anticoagulant mugukora plasma ikungahaye kuri platine (PRP) hamwe na PRP Sisitemu yo gutunganya amaraso adasanzwe.
Anticoagulant Citrate Dextrose Umuti, bakunze kwita ACD-A cyangwa Umuti A ni igisubizo kitari pyrogene, sterile.Iyi element ikoreshwa nka anticoagulant mugukora plasma ikungahaye kuri platine (PRP) hamwe na PRP Sisitemu yo gutunganya amaraso adasanzwe.Anticoagulants ishingiye kuri citrate ikoresha ubushobozi bwa citrate ion yo gukuramo calcium ionisiyumu iboneka mumaraso kugirango irinde kwanduza amaraso no gukora calcium-citrate idafite ionisiyoneri.
Igicuruzwa cyonyine kirwanya anticagulant ikigo cy’Amerika gishinzwe ibiryo n’ibiyobyabwenge cyemeje gukoreshwa mu gutegura PRP muri sisitemu zitandukanye za PRP ni ACD-A.Nk’uko ubushakashatsi bwakozwe mu mwaka wa 2016 kuri PRP bwabonetse hamwe na anticagulants zitandukanye n'ingaruka zabyo ku myitwarire ya selile ya mesenchymal tromal selile in vitro na platelet, hari ibisubizo byiza mu ikoreshwa rya PRP mu gusana ingirangingo z'imitsi.
Kubitandukanya platine birasabwa gusimbuza sodium citrate isanzwe ya Acide Citrate Dextrose (ACD-A) kuko uburyo bwo kwigunga busaba intambwe nyinshi zo gukaraba.Amashanyarazi arahagaze neza kuri 37C mugihe cyo kuzunguruka, ariko kuzunguruka mubushyuhe bwicyumba (25 C) nabyo bikora neza.Kugabanya pH binyuze muri ACD-A (igera hafi ya 6.5) ifasha kubangamira imikorere yimisemburo ya trombine isigaye mu miyoboro ya platine, kandi ikagira uruhare mukubungabunga muri rusange morphologie ya platine mugihe ihindura imikorere kuri minima.Mubisanzwe ugomba guhagarika platine kuri Tyrode Buffer ikwiye (pH 7.4) kugirango ugarure imikorere yabo.ACD ifite ibyiza byinshi mugihe cyo kubungabunga platine
Iyo ACD yakoreshejwe, ibisubizo byerekanaga umusaruro mwinshi wa platine mumaraso muri rusange.Ariko, ikoreshwa rya EDTA ryanashishikarije gukura mubunini bwa platine nyuma yintambwe yo gukusanya amaraso kugirango babone PRP.Ibikurikira, ikoreshwa rya ACD ryatumye habaho kwiyongera kwa selile ya mesenchymal tromal.Niyo mpamvu, hanzuwe ko imiti igabanya ubukana harimo ACD-A igira uruhare runini mugutegura PRP no gufasha kunoza inzira kuburyo bugaragara.
