Plasma ikungahaye kuri plasma itera angiogenez mu mbeba zishobora guteza imbere umusatsi

Plasma ikungahaye kuri plasma (PRP) ni autologique yibanda kuri platine yabantu muri plasma.Binyuze mu kwangirika kwa alpha granules muri platine, PRP irashobora gusohora ibintu bitandukanye bikura, harimo gukura kwa platine (PDGF), gukura kwamaraso ya endoteliyale (VEGF), gukura kwa fibroblast (FGF), gukura kwa hepatocyte (HGF), no guhindura ibintu bikura (TGF), byanditswe kugirango bitangire gukira ibikomere no guteza imbere ikwirakwizwa no guhindura ingirabuzimafatizo za endoteliyale na pericyite mu mikurire ya endoteliyale.

Uruhare rwa PRP mu kuvura imikurire y’imisatsi rwagaragaye mu bushakashatsi bwinshi buherutse gukorwa.Uebel n'abandi.basanze gukura kwa platel plasma byongera umusaruro wibice bya follicular mugikorwa cyo kubaga umusatsi wumugabo.Ibikorwa biherutse gukorwa byerekanye ko PRP yongerera ikwirakwizwa rya papilla dermal dermal kandi igatera kwihuta kwa telogene-kuri-anagen ukoresheje vivo no muri moderi ya vitro.Ubundi bushakashatsi bwerekanye ko PRP iteza imbere imisatsi yumusatsi kandi ikagabanya cyane igihe cyo kumera umusatsi.

Byombi PRP na platel-ikennye plasma (PPP) ikubiyemo kuzuza byuzuye poroteyine za coagulation.Muri ubu bushakashatsi, ubushakashatsi bwakozwe kuri PRP na PPP ku mikurire yimisatsi ku mbeba C57BL / 6.Igitekerezo cyari uko PRP yagize ingaruka nziza kumikurire yuburebure bwumusatsi no kongera umubare wimisatsi.

Inyamaswa zigeragezwa

Imbeba 50 z'umugabo C57BL / 6 zifite ubuzima bwiza (ibyumweru 6, 20 ± 2 g) zabonetse mu kigo cy’inyamaswa za Laboratoire, kaminuza isanzwe ya Hangzhou (Hangzhou, Ubushinwa).Amatungo yagaburiwe ibiryo bimwe kandi akabungabungwa ahantu hahoraho munsi yumucyo wa 12: 12-h.Nyuma yicyumweru 1 cyo kumenyera, imbeba zagabanyijwemo amatsinda atatu: itsinda rya PRP (n = 10), itsinda rya PPP (n = 10), nitsinda rishinzwe kugenzura (n = 10).

Porotokole y’ubushakashatsi yemejwe na komite ishinzwe imyitwarire y’inzego z’ubushakashatsi ku nyamaswa hakurikijwe amategeko y’ubushakashatsi bw’inyamaswa n’amategeko agenga Ubushinwa.

Gupima uburebure bwimisatsi

Ku minsi 8, 13, na 18 nyuma yo guterwa bwa nyuma, imisatsi 10 muri buri mbeba yatoranijwe ku bushake ahantu hagenewe.Ibipimo by'uburebure bw'imisatsi byakorewe mu mirima itatu hakoreshejwe microscope ya electron, kandi impuzandengo yabo yagaragajwe nka milimetero.Umusatsi muremure cyangwa wangiritse washyizwemo.

Hematoxyline na eosine (HE) irangi

Urugero rwuruhu rwa Dorsal rwacukuwe muminsi 18 nyuma yo guterwa inshuro ya gatatu.Noneho ingero zashyizwe muri 10% zidafite aho zibogamiye, zashyizwe muri paraffine, hanyuma zicamo 4 mm.Ibice byatekeshejwe saa yine za deparaffinisation kuri 65 ° C, bigashyirwa muri Ethanol ya gradient, hanyuma bigashyirwa hamwe na hematoxyline muminota 5.Nyuma yo gutandukanywa na alcool ya hydrochloric 1%, ibice byashyizwe mumazi ya amoniya, byandujwe na eosine, hanyuma byogejwe namazi yatoboye.Hanyuma, ibice byashizwemo umwuma na Ethanol ya gradient, bisukurwa na xylene, bigashyirwaho resin idafite aho ibogamiye, kandi byarebwaga hakoreshejwe microscopi yoroheje (Olympus, Tokiyo, Ubuyapani).