Umuyoboro umwe wa kirimbuzi Gel Gutandukanya Tube-CPT Tube

Imirasire imwe ya kirimbuzi Gel Gutandukanya Tube-CPT Tube Yerekana Ishusho

Ibisobanuro bigufi:

Ikoreshwa mugutandukanya monocytes mumaraso yose.

Ikoreshwa cyane cyane mumikorere yumubiri wa lymphocyte nka HLA, leukemia isigaye ya gene hamwe nubuvuzi bwikingira.

Ibicuruzwa birambuye

Ibicuruzwa

Niki CPT Tube

Umuyoboro umwe wa selile seleration tube (CPT tube) wongeyeho hypaque, anticoagulant na gel yo gutandukana.Lymphocytes na selile mononuclear birashobora gutandukanywa byoroshye mumaraso yose hamwe na centrifugation yintambwe imwe mugihe ukoresheje gel idasanzwe yo gutandukanya selile.Ikoreshwa cyane cyane mugutahura imikorere yumubiri wa lymphocyte, HLA cyangwa leukemia isigaye ya gene hamwe nubuvuzi bwikingira.Itanga uburyo busanzwe bwo gukuramo intambwe imwe yo gukuramo monocytes yo kwisuzumisha kwa muganga no gupima immunotherapy.

Imikorere y'ibicuruzwa

1) Ingano: 13 * 100mm, 16 * 125mm;

2) Ingano yinyongera: 0.1ml, 135usp;

3) Umubare w'amaraso: 4ml, 8ml;

4) Ubuzima bwa Shelf: amezi 24 uhereye umunsi yakorewe;

5) Ububiko:Ubike kuri 18-25℃.

IbicuruzwaIbyiza

1) Bikora neza, byukuri kandi bifite umutekano;

2) Hamwe na Ficoll Hypaque yubatswe, anticoagulant na gel yo gutandukana, selile monon nuclear itandukanijwe namaraso yose hamwe na centrifugation yintambwe imwe.

3) Tekinoroji yo gutandukanya selile.

4) Urukuta rw'imbere bionic membrane tekinoroji yo gutunganya;

5) Igipimo cyo gukira kwa monocytes kirenga 90%, ubuziranenge burenga 95%, naho kubaho birarenze 99%

Ibintu bikeneye kwitabwaho

Ingingo zikurikira zigomba kwitonderwa:

1) Mugihe ukora igeragezwa ryimico yingirabuzimafatizo, witondere imikorere ya aseptic, uhindure reagent (igisubizo cyo gutandukana, igisubizo cyo gukaraba, nibindi) nibikoresho.Iki gikorwa kigomba gukorwa ninzobere kugirango zemeze guhuza ibikorwa.

2) Ubushyuhe bwa Centrifugation mubusanzwe mubushyuhe bwicyumba (2 ~ 25 ℃).

3) Mubisanzwe iyo utandukanije ingirabuzimafatizo (PBMC) na Ficoll, ubwinshi bwingirangingo zamaraso zitukura ni buto, bushobora guhindura ukuri kwubushakashatsi.Lysate rero irashobora gukoreshwa (bamwe bakeneye guhindurwa), kugenzura igihe cya lysis no kwirinda kugira ingaruka kumikorere ya selile monone.

4) Witondere abou re-centrifugation ishobora kwikuba kabiri.

Mbere: CTAD Kumenya Tube

Ibikurikira: Amaraso atukura